Attenuated hepatitis A virus: genetic determinants of adaptation to growth in MRC-5 cells.

doi:10.1128/JVI.68.1.148-157.1994

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Article

Attenuated hepatitis A virus: genetic determinants of adaptation to growth in MRC-5 cells.

Journal of virology - 1 Jan 1994

Funkhouser A W, Purcell R H, D'Hondt E, Emerson S U

Abstract excerpt

A live candidate hepatitis A virus vaccine, developed from the HM-175 strain and adapted to growth in primary African green monkey kidney (AGMK) cells, was adapted to growth in MRC-5 cells. The nucleotide sequence of the MRC-5 cell-adapted virus was determined and compared with the known sequence of the AGMK cell-adapted virus. Thirteen unique mutations, which occurred during passage in MRC-5 cells, were...

Topics

Adaptation, Physiological
Animals
Blotting, Northern
Cells, Cultured
Cloning, Molecular
DNA, Complementary
Haplorhini
Hepatitis A Vaccines
Hepatovirus
Humans
Mutation

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Nucleic Acid Hybridization

Phenotype

Polymerase Chain Reaction

RNA, Messenger

Radioimmunoassay

Sequence Analysis, DNA

Serial Passage

Source

Open in PubMed Open full text

Identifiers

PMID: 8254724
DOI: 10.1128/JVI.68.1.148-157.1994
PMCID: PMC236273

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-8254724-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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