Article
Typing of serum-soluble HLA-B27 antigen by ELISA.
Tissue antigens - 1 Jul 1993
Pouletty P, Chang C, Kalil J, Atwood E, Ferrone S, Shimizu B, Howson W, Mazaheri R, Del Villano B, Grumet C
Abstract excerpt
An ELISA using serum as soluble HLA antigen source was developed for HLA-B27 typing. Two sandwich assays were run in parallel. The first assay utilized a monoclonal antibody (mAb) reacting with a determinant expressed by both HLA-B7 and B27 antigens; the other assay utilized a mAb reactive with HLA-B7 antigens but not with HLA-B27 antigens. After incubation with serum samples, bound HLA antigen was detected using...
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