Typing of serum-soluble HLA-B27 antigen by ELISA.

doi:10.1111/j.1399-0039.1993.tb02160.x

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Article

Typing of serum-soluble HLA-B27 antigen by ELISA.

Tissue antigens - 1 Jul 1993

Pouletty P, Chang C, Kalil J, Atwood E, Ferrone S, Shimizu B, Howson W, Mazaheri R, Del Villano B, Grumet C

Abstract excerpt

An ELISA using serum as soluble HLA antigen source was developed for HLA-B27 typing. Two sandwich assays were run in parallel. The first assay utilized a monoclonal antibody (mAb) reacting with a determinant expressed by both HLA-B7 and B27 antigens; the other assay utilized a mAb reactive with H...

Topics

Algorithms
Antibodies, Monoclonal
Enzyme-Linked Immunosorbent Assay
HLA-B27 Antigen
HLA-B7 Antigen
Humans
Phenotype
Reproducibility of Results
Software

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