Article
Epitope analysis of an HLA-B27-derived synthetic peptide: a possible approach to analyzing HLA class I antigens.
Clinical and experimental rheumatology - 1 Jan 2000
Zhao Y P, Shi B J, Deng H K, Yu D T, Hamachi M, Hamachi T, Zhao D F, Tang S R, Tsang J C, Park M S
Abstract excerpt
A monoclonal antibody, F3H7, was generated by immunizing mice with a synthetic peptide corresponding to residues 63-84 of the B*2705 allele of the HLA-B27 antigens. The reactive epitope and the contact residues on the peptide were localized by ELISA using a large panel of overlapping peptides as...
Topics
- Alleles
- Amino Acid Sequence
- Amino Acids
- Animals
- Antibodies, Monoclonal
- Enzyme-Linked Immunosorbent Assay
- Epitopes
- HLA-B27 Antigen
- Histocompatibility Antigens Class I
- Lymphocytes
- Mice
- Mice, Inbred BALB C
- Molecular Sequence Data
