Article
Improved flow cytometric method for HLA-B27 typing.
Annals of clinical biochemistry - 1 Nov 1992
Janssen W C, Rouwen J A, Hoffmann J J
Abstract excerpt
HLA-B27 is a cell marker of clinical interest because of its high association with certain diseases. The HLA-B27 antigen was detected on lymphocytes using a monoclonal antibody in an indirect immuno-fluorescence assay using a fluorescence flow cytometer. The considerable crossreaction of the monoclonal antibody with the HLA-B7 antigen was effectively suppressed by masking it by means of human anti-HLA-B7...
Topics
- Cross Reactions
- Flow Cytometry
- Fluorescent Antibody Technique
- HLA-B27 Antigen
- HLA-B7 Antigen
- Humans
- Phenotype
