Article
Detection of HLA-specific IGG antibodies using single recombinant HLA alleles: the MonoLISA assay.
Transplantation - 15 Aug 2000
Barnardo M C, Harmer A W, Shaw O J, Ogg G S, Bunce M, Vaughan R W, Morris P J, Welsh K I
Abstract excerpt
BACKGROUND: Because of the presence of confounding antigens, the assignment of HLA antibody specificity is difficult in highly sensitized patients, and the definition of an acceptable HLA mismatch requires a significant workload per patient. We describe a new ELISA method, monoLISA, for detection of immunoglobulin (Ig)G HLA antibody using single recombinant HLA class I monomers bound to microtiter plates....
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.