Article
Typing of a panel of soluble HLA class I antigens by enzyme-linked immunosorbent assay.
Human immunology - 1 Jul 1994
Pouletty C, Mercier I, Glanville L, Tomavo N, Igoudin L, Pouletty P, Buelow R
Abstract excerpt
Two ELISA assays were developed to test the reactivity of soluble sHLAs with anti-HLA class I mAbs of IgG or IgM isotype. A panel of 40 different alleles of sHLA antigens was produced using 57 lymphoblastoid B-cell lines, which had been generated from class-I-phenotyped PBLs. Using 14 mAbs, the expression of 13 different sHLA antigens (sHLA-A2, -A3, -A11, -A24, -A29, -B7, -B8, -B13, -B14, -B27, -B44, -B57, and...
Topics
- Alleles
- Antibodies, Monoclonal
- B-Lymphocytes
- Cell Transformation, Viral
- Cytotoxicity Tests, Immunologic
- Enzyme-Linked Immunosorbent Assay
- Histocompatibility Antigens Class I
- Humans
- Immunoglobulin G
- Immunoglobulin M