Article
The HYP2 gene of Saccharomyces cerevisiae is essential for aerobic growth: characterization of different isoforms of the hypusine-containing protein Hyp2p and analysis of gene disruption mutants.
Molecular & general genetics : MGG - 1 Nov 1993
Wöhl T, Klier H, Ammer H, Lottspeich F, Magdolen V
Abstract excerpt
In Saccharomyces cerevisiae, hypusine-containing proteins are encoded by two closely related genes, HYP1 and HYP2, which are regulated reciprocally by oxygen and heme. We have purified the aerobically expressed hypusine-containing proteins from yeast. The three proteins detected (two isoforms, which differ in their pI values, and a degradation product thereof, lacking the N-terminal 10 amino acid residues) are...
Topics
- Aerobiosis
- Anaerobiosis
- Chromatography, High Pressure Liquid
- Electrophoresis, Gel, Two-Dimensional
- Fungal Proteins
- Genes, Fungal
- Lysine
- Mutation
- Saccharomyces cerevisiae