Article
Biochemical characterization of Hpa2 and Hpa3, two small closely related acetyltransferases from Saccharomyces cerevisiae.
The Journal of biological chemistry - 26 Jul 2013
Sampath Vinaya, Liu Bingsheng, Tafrov Stefan, Srinivasan Madhusudhan, Rieger Robert, Chen Emily I, Sternglanz Rolf
Abstract excerpt
Based on their sequences, the Saccharomyces cerevisiae Hpa2 and Hpa3 proteins are annotated as two closely related members of the Gcn5 acetyltransferase family. Here, we describe the biochemical characterization of Hpa2 and Hpa3 as bona fide acetyltransferases with different substrate specificities. Mutational and MALDI-TOF analyses showed that Hpa3 translation initiates primarily from Met-19 rather than the...
Topics
- Acetylation
- Acetyltransferases
- Amino Acid Sequence
- Amino Acids
- Amino-Acid N-Acetyltransferase
- Codon, Initiator
- Electrophoresis, Polyacrylamide Gel
- High Mobility Group Proteins
- Histone Acetyltransferases
- Histones
- Isoenzymes
- Lysine
- Methionine