Article
A rare tRNA-Arg(CCU) that regulates Ty1 element ribosomal frameshifting is essential for Ty1 retrotransposition in Saccharomyces cerevisiae.
Genetics - 1 Oct 1993
Kawakami K, Pande S, Faiola B, Moore D P, Boeke J D, Farabaugh P J, Strathern J N, Nakamura Y, Garfinkel D J
Abstract excerpt
Translation of the yeast retrotransposon Ty1 TYA1(gag)-TYB1(pol) gene occurs by a +1 ribosomal frameshifting event at the sequence CUU AGG C. Because overexpression of a low abundance tRNA-Arg(CCU) encoded by the HSX1 gene resulted in a reduction in Ty1 frameshifting, it was suggested that a translational pause at the AGG-Arg codon is required for optimum frameshifting. The present work shows that the absence of...
Topics
- Base Sequence
- DNA Primers
- DNA Transposable Elements
- Diploidy
- Frameshift Mutation
- Gene Expression Regulation, Fungal
- Genes, Fungal
- Genes, gag
- Genes, pol
- Genotype
- Molecular Sequence Data