Article
The yeast retrotransposon Ty5 uses the anticodon stem-loop of the initiator methionine tRNA as a primer for reverse transcription.
RNA (New York, N.Y.) - 1 Jul 1999
Ke N, Gao X, Keeney J B, Boeke J D, Voytas D F
Abstract excerpt
Retrotransposons and retroviruses replicate by reverse transcription of an mRNA intermediate. Most retroelements initiate reverse transcription from a host-encoded tRNA primer. DNA synthesis typically extends from the 3'-OH of the acceptor stem, which is complementary to sequences on the retroelement mRNA (the primer binding site, PBS). However, for some retrotransposons, including the yeast Ty5 elements,...
Topics
- Amino Acid Sequence
- Anticodon
- Base Sequence
- Genetic Complementation Test
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Nucleic Acid Conformation
- Plasmids
- RNA
- RNA, Transfer, Met
- Retroelements
- Saccharomyces cerevisiae