Phenotype analysis of cycling and postcycling thymocytes: evaluation of detection methods for BrdUrd and surface proteins.

doi:10.1002/cyto.990140708

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Article

Phenotype analysis of cycling and postcycling thymocytes: evaluation of detection methods for BrdUrd and surface proteins.

Cytometry - 1 Oct 1993

Penit C, Vasseur F

Abstract excerpt

We present a comparison of two different methods for simultaneous detection of bromodeoxyuridine and cell surface markers. Both methods use enzymatic generation of single-strand DNA with nuclease. The biological system used is the murine thymus, in which in vivo DNA synthetizing cells were labeled by injection of BrdUrd and analyzed at different time points after the nucleoside pulse. The surface proteins...

Topics

Animals
Bromodeoxyuridine
CD4 Antigens
CD8 Antigens
Cell Differentiation
DNA
DNA Replication
Dactinomycin
Deoxyribonuclease I
Flow Cytometry
Fluorescent Antibody Technique

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Humans

Membrane Proteins

Mice

Mice, Inbred C57BL

Phenotype

Receptors, Antigen, T-Cell

T-Lymphocyte Subsets

Source

Open in PubMed

Identifiers

PMID: 8243204
DOI: 10.1002/cyto.990140708

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-8243204-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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