Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Simultaneous three-color analysis of the surface phenotype and DNA-RNA quantitation using 7-amino-actinomycin D and pyronin Y.

Journal of immunological methods - 9 Jun 1995

Toba K, Winton E F, Koike T, Shibata A

Abstract excerpt

We developed an improved technique that permits simultaneous DNA and RNA quantitation by a flow cytofluorometry using 7-amino-actinomycin D (7AAD) and pyronin Y (PY), respectively. Detailed cell cycle analyses based upon the cellular DNA/RNA levels were performed using cells suspended in a buffer containing 0.004% saponin. This method preserved the light scattering properties of human peripheral blood cells, thus...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Cell Cycle
Share to Cell Line
Share to DNA

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Flow Cytometry

Share to HLA-DR Antigens

Share to In Vitro Techniques

Share to Lymphocytes

Share to Phenotype

Source

Identifiers

PMID: 7540639
DOI: 10.1016/0022-1759(95)00050-k

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Simultaneous three-color analysis of the surface phenotype and DNA-RNA quantitation using 7-amino-actinomycin D and pyronin Y.PMID 7540639Read ↗

Flow cytometric analysis of live cell proliferation and phenotype in populations with low viability.Semantic proximity Read ↗

Novel approach for simultaneous evaluation of cell phenotype, apoptosis, and cell cycle using multiparameter flow cytometry.Semantic proximity Read ↗

Polychromatic (eight-color) slide-based cytometry for the phenotyping of leukocyte, NK, and NKT subsets.Semantic proximity Read ↗

OMIP-067: 28-Color Flow Cytometry Panel to Evaluate Human T-Cell Phenotype and Function.Semantic proximity Read ↗

Phenotype analysis of cycling and postcycling thymocytes: evaluation of detection methods for BrdUrd and surface proteins.Semantic proximity Read ↗

Cytofluorometric Identification of Two Populations of Double Positive (CD4+,CD8+) T Lymphocytes in Human Peripheral BloodSemantic proximity Read ↗

OMIP-006: phenotypic subset analysis of human T regulatory cells via polychromatic flow cytometry.Semantic proximity Read ↗

[Value of phenotyping by cytofluorometry in hematology and clinical immunopathology].Semantic proximity Read ↗

A novel and easy F xCycle ™ violet based flow cytometric method for simultaneous assessment of DNA ploidy and six‐color immunophenotypingSemantic proximity Read ↗

Phenotype transition of CD4+ T cells from CD45RA to CD45R0 is accompanied by cell activation and proliferation.Semantic proximity Read ↗

One-tube triple staining method for flow cytometric analysis of DNA ploidy and phenotypic heterogeneity of human solid tumors using single laser excitation.Semantic proximity Read ↗

Beyond six colors: a new era in flow cytometry.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.