Article
Simultaneous three-color analysis of the surface phenotype and DNA-RNA quantitation using 7-amino-actinomycin D and pyronin Y.
Journal of immunological methods - 9 Jun 1995
Toba K, Winton E F, Koike T, Shibata A
Abstract excerpt
We developed an improved technique that permits simultaneous DNA and RNA quantitation by a flow cytofluorometry using 7-amino-actinomycin D (7AAD) and pyronin Y (PY), respectively. Detailed cell cycle analyses based upon the cellular DNA/RNA levels were performed using cells suspended in a buffer...
Topics
- Antigens, Differentiation
- Cell Cycle
- Cell Line
- DNA
- Dactinomycin
- Flow Cytometry
- Fluorescent Dyes
- HLA-DR Antigens
- Humans
- Immunologic Techniques
- In Vitro Techniques
- Lymphocytes
- Phenotype
- Pyronine
- RNA
- Receptors, Interleukin-2
- Receptors, Transferrin
- Saponins