Assessment of cell proliferation by 5-bromodeoxyuridine (BrdU) labeling for multicolor flow cytometry.

doi:10.1002/0471142956.cy0731s40

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Article

Assessment of cell proliferation by 5-bromodeoxyuridine (BrdU) labeling for multicolor flow cytometry.

Current protocols in cytometry - 1 Apr 2007

Rothaeusler Kristina, Baumgarth Nicole

Abstract excerpt

Cell proliferation assays are used for a large variety of applications in the life sciences. This unit describes a flow-cytometry-based method that uses BrdU labeling in conjunction with multiple fluorescently labeled cell surface markers, allowing extensive phenotypic characterization of dividing cells in addition to assessment of their frequency. BrdU labeling has the advantage of constituting a nonradioactive...

Topics

Bromodeoxyuridine
Cell Division
Cell Membrane Permeability
DNA
Flow Cytometry
Fluorescent Dyes
Image Cytometry
Immunotoxins
Indicators and Reagents
Phenotype
Ribosome Inactivating Proteins, Type 1
Saporins

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Open in PubMed

Identifiers

PMID: 18770853
DOI: 10.1002/0471142956.cy0731s40

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