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Determining Cell Cycle Stages by Flow Cytometry

1 Jan 1999

Abstract excerpt

This unit describes assays used to determine the distribution of a population of cells to the different stages of the cell cycle as analyzed by flow cytometry. Staining the DNA with different fluorescent dyes, propidium iodide or DAPI, is one of the most direct ways of staging the cells based on DNA content. These staining procedures can be used with fixed cells or detergent-permeabilized cells. Alternatively,...

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PMID: 18228389
DOI: 10.1002/0471143030.cb0804s01

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