Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Use of a gene encoding a suppressor tRNA as a reporter of transcription: analyzing the action of the Nun protein of bacteriophage HK022.

Proceedings of the National Academy of Sciences of the United States of America - 1 Nov 1993

Sloan S B, Weisberg R A

Abstract excerpt

The Nun protein of phage HK022 blocks the expression of genes that lie downstream of the nut sites of phage lambda. Nun is believed to act by promoting premature termination of transcription at or near these sites. To test this hypothesis and to facilitate mapping the sites of termination, we inserted a gene encoding a suppressor tRNA immediately downstream of the lambda nutL site and determined the effect of Nun...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Base Sequence
Share to Cloning, Molecular

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Escherichia coli

Share to Gene Expression

Share to Genotype

Share to Molecular Sequence Data

Share to Plasmids

Share to Restriction Mapping

Source

Open in PubMed Open full text

Identifiers

PMID: 8234323
DOI: 10.1073/pnas.90.21.9842
PMCID: PMC47668

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Use of a gene encoding a suppressor tRNA as a reporter of transcription: analyzing the action of the Nun protein of bacteriophage HK022.PMID 8234323Read ↗

Phage HK022 Nun protein represses translation of phage lambda N (transcription termination/translation repression).Semantic proximity Read ↗

Lambda nutR mutations convert HK022 Nun protein from a transcription termination factor to a suppressor of termination.Semantic proximity Read ↗

Coliphage HK022 Nun protein inhibits RNA polymerase translocation.Semantic proximity Read ↗

Role of E.coli NusA in phage HK022 Nun-mediated transcription termination.Semantic proximity Read ↗

Overexpression of phage HK022 Nun protein is toxic for Escherichia coli.Semantic proximity Read ↗

The nut site of bacteriophage lambda is made of RNA and is bound by transcription antitermination factors on the surface of RNA polymerase.Semantic proximity Read ↗

The antiterminator RNA of phage HK022.Semantic proximity Read ↗

The Y39A mutation of HK022 Nun disrupts a boxB interaction but preserves termination activity.Semantic proximity Read ↗

N-mediated transcription antitermination in lambdoid phage H-19B is characterized by alternative NUT RNA structures and a reduced requirement for host factors.Semantic proximity Read ↗

Escherichia coli nusG mutations that block transcription termination by coliphage HK022 Nun protein.Semantic proximity Read ↗

Bacteriophage lambda N-dependent transcription antitermination. Competition for an RNA site may regulate antitermination.Semantic proximity Read ↗

Translational repression by a transcriptional elongation factor.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.