Article
Use of a gene encoding a suppressor tRNA as a reporter of transcription: analyzing the action of the Nun protein of bacteriophage HK022.
Proceedings of the National Academy of Sciences of the United States of America - 1 Nov 1993
Sloan S B, Weisberg R A
Abstract excerpt
The Nun protein of phage HK022 blocks the expression of genes that lie downstream of the nut sites of phage lambda. Nun is believed to act by promoting premature termination of transcription at or near these sites. To test this hypothesis and to facilitate mapping the sites of termination, we ins...
Topics
- Bacteriophage lambda
- Base Sequence
- Blotting, Northern
- Cloning, Molecular
- Coliphages
- Escherichia coli
- Gene Expression
- Genes, Suppressor
- Genes, Viral
- Genotype
- Molecular Sequence Data
- Nucleic Acid Conformation
- Plasmids
- RNA, Transfer, Gly
- Restriction Mapping
- Suppression, Genetic
- Transcription Factors
- Transcription, Genetic