Article
Lambda nutR mutations convert HK022 Nun protein from a transcription termination factor to a suppressor of termination.
Journal of molecular biology - 20 Apr 1990
Robledo R, Gottesman M E, Weisberg R A
Abstract excerpt
The Nun protein of the lambdoid phage HK022 blocks lambda growth by terminating transcription at (or near) the lambda nut sites. An HK022 lysogen carrying a fusion of the lambda pR promoter and nutR site to a gal operon that lacks its own promoter is, therefore, Gal-. To characterize the target of Nun action, spontaneous Gal+ revertants of this strain were isolated and characterized. Two cis-acting mutations are...
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