Article
The nut site of bacteriophage lambda is made of RNA and is bound by transcription antitermination factors on the surface of RNA polymerase.
Genes & development - 1 Nov 1991
Nodwell J R, Greenblatt J
Abstract excerpt
The boxA and boxB components of the lambda nut site are important for transcriptional antitermination by the phage N protein. We show here that boxA and boxB RNA in N-modified transcription complexes are inaccessible to ribonucleases and have altered sensitivity to dimethylsulfate. N and NusA suffice to weakly protect boxB, independently of boxA and other factors. However, efficient protection of the entire nut...
Topics
- Bacteriophage lambda
- Base Sequence
- DNA-Directed RNA Polymerases
- Escherichia coli
- Genes, Viral
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- RNA, Viral
- Sulfuric Acid Esters
- Transcription Factors