Article
Critical factors governing the difference in antizyme-binding affinities between human ornithine decarboxylase and antizyme inhibitor.
PloS one - 28 Apr 2011
Liu Yen-Chin, Liu Yi-Liang, Su Jia-Yang, Liu Guang-Yaw, Hung Hui-Chih
Abstract excerpt
Both ornithine decarboxylase (ODC) and its regulatory protein, antizyme inhibitor (AZI), can bind with antizyme (AZ), but the latter has a higher AZ-binding affinity. The results of this study clearly identify the critical amino acid residues governing the difference in AZ-binding affinities between human ODC and AZI. Inhibition experiments using a series of ODC mutants suggested that residues 125 and 140 may be...
Topics
- Amino Acid Sequence
- Binding Sites
- Enzyme Inhibitors
- Humans
- Models, Molecular
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Ornithine Decarboxylase
- Ornithine Decarboxylase Inhibitors
- Protein Binding
- Proteins
- Solvents
- Static Electricity