Working with Phage P22.

doi:10.1101/pdb.prot107850

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Article

Working with Phage P22.

Cold Spring Harbor protocols - 3 Oct 2022

Figueroa-Bossi Nara, Balbontín Roberto, Bossi Lionello

Abstract excerpt

Transduction experiments in Escherichia coli and Salmonella are usually performed with virulent phage variants. A widely used P1 mutant, called P1 vir, carries one or more uncharacterized mutations that prevent formation of lysogens. In the case of P22, by far the most frequently used variant is named P22 HT105/1 int-201 This phage has a high transducing (HT) frequency due to a mutant nuclease with lower...

Topics

Bacteriophage P22
Escherichia coli
Mutation
Salmonella Phages
Transduction, Genetic

Source

Open in PubMed

Identifiers

PMID: 35960630
DOI: 10.1101/pdb.prot107850

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026.

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