Approaches to half-tetrad analysis in bacteria: recombination between repeated, inverse-order chromosomal sequences.

Abstract excerpt

In standard bacterial recombination assays, a linear fragment of DNA is transferred to a recipient cell and, at most, a single selected recombinant type is recovered from each merozygote. This contrasts with fungal systems, for which tetrads allow recovery of all meiotic products, including both ultimate recombinant products of an apparent single act of recombination. We have developed a bacterial recombination...

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