Article
A robotics-assisted procedure for large scale cystic fibrosis mutation analysis.
Human mutation - 1 Jan 1994
DeMarchi J M, Richards C S, Fenwick R G, Pace R, Beaudet A L
Abstract excerpt
We describe a convenient, efficient, semiautomated protocol for assaying large numbers of DNA samples for over 20 mutations causing cystic fibrosis. The protocol uses the following: (1) a programmable robotic workstation to perform rapid pipetting and dot-blotting operations, (2) an allele-specif...
Topics
- Base Sequence
- Cystic Fibrosis
- DNA Mutational Analysis
- Exons
- Humans
- Molecular Sequence Data
- Mutation
- Polymerase Chain Reaction
- Robotics