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Article

A robotics-assisted procedure for large scale cystic fibrosis mutation analysis.

Human mutation - 1 Jan 1994

DeMarchi J M, Richards C S, Fenwick R G, Pace R, Beaudet A L

Abstract excerpt

We describe a convenient, efficient, semiautomated protocol for assaying large numbers of DNA samples for over 20 mutations causing cystic fibrosis. The protocol uses the following: (1) a programmable robotic workstation to perform rapid pipetting and dot-blotting operations, (2) an allele-specific oligonucleotide hybridization in a single water bath without correcting for G+C content of oligonucleotides, and (3)...

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Identifiers

PMID: 7866408
DOI: 10.1002/humu.1380040409

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