Site-directed mutagenesis of nitrate reductase from Aspergillus nidulans. Identification of some essential and some nonessential amino acids among conserved residues.

doi:10.1074/jbc.270.12.6644

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Article

Site-directed mutagenesis of nitrate reductase from Aspergillus nidulans. Identification of some essential and some nonessential amino acids among conserved residues.

The Journal of biological chemistry - 24 Mar 1995

Garde J, Kinghorn J R, Tomsett A B

Abstract excerpt

Nitrate reductase is a multiredox enzyme possessing three functional domains associated with the prosthetic groups FAD, heme iron, and molybdopterin. In Aspergillus nidulans, it is encoded by the niaD gene. A homologous transformation system has been used whereby a major deletion at the niiAniaD...

Topics

Aspergillus nidulans
Base Sequence
Molecular Sequence Data
Mutagenesis, Site-Directed
NADP
Nitrate Reductase
Nitrate Reductases
Phenotype
Structure-Activity Relationship

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