Transformation of Botrytis cinerea with the nitrate reductase gene (niaD) shows a high frequency of homologous recombination.

doi:10.1007/s002940050261

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Article

Transformation of Botrytis cinerea with the nitrate reductase gene (niaD) shows a high frequency of homologous recombination.

Current genetics - 1 Aug 1997

Levis C, Fortini D, Brygoo Y

Abstract excerpt

The nitrate reductase (niaD) gene was isolated from the phytopathogenic ascomycete Botrytis cinerea using a probe obtained by a polymerase chain reaction (PCR) with degenerate oligonucleotides corresponding to domains conserved among three fungal nitrate reductases. The B. cinerea niaD gene encod...

Topics

Cloning, Molecular
Fusarium
Mitosporic Fungi
Mutation
Nitrate Reductase
Nitrate Reductases
Recombinant Proteins
Recombination, Genetic
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Transformation, Genetic

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