Article
Homologous transformation of Cephalosporium acremonium with the nitrate reductase-encoding gene (niaD).
Gene - 15 Jun 1990
Whitehead M P, Gurr S J, Grieve C, Unkles S E, Spence D, Ramsden M, Kinghorn J R
Abstract excerpt
We report the development of a homologous transformation system for Cephalosporium acremonium using the niaD gene of the nitrate assimilation (NA) pathway. Mutants in the NA pathway were selected on the basis of chlorate resistance by conventional means. Screening procedures were developed to differentiate between nitrate reductase apoprotein structural gene mutants (niaD) and molybdenum cofactor gene mutants...
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