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Article

Isolation and characterization of experimentally induced, aflatoxin biosynthetic pathway deletion mutants of Aspergillus parasiticus.

Applied microbiology and biotechnology - 1 Jun 1999

Cary J W, Barnaby N, Ehrlich K C, Bhatnagar D

Abstract excerpt

A plasmid vector (pDEL2) was engineered for the purpose of introducing a deletion within the aflatoxin (AF) biosynthetic gene cluster of Aspergillus parasiticus. The vector was constructed by PCR amplification of a region of the AF gene cluster from an A. parasiticus isolate that had undergone an aberrant recombinational event during transformation with a nor A-niaD gene disruption vector. This recombinational...

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PMID: 10422228
DOI: 10.1007/s002530051466

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