Article
Nuclear genes that promote splicing of group I introns in the chloroplast 23S rRNA and psbA genes in Chlamydomonas reinhardtii.
The Plant journal : for cell and molecular biology - 1 Nov 2002
Li Fei, Holloway Stephen P, Lee Jaesung, Herrin David L
Abstract excerpt
Single nucleotide substitutions were made in the core helices P4, P6, and P7, and in the metal-binding GAAA motif in the J4/5 region of the chloroplast group I rRNA intron of Chlamydomonas reinhardtii, Cr.LSU. In vitro assays showed that these substitutions had surprisingly strong effects on Cr.LSU self-splicing; however, splicing of all but the P6 mutations could be at least partially recovered by increasing the...
Topics
- Animals
- Base Sequence
- Cell Nucleus
- Chlamydomonas reinhardtii
- Genes, Dominant
- Genes, Plant
- Introns
- Mutation
- Nucleic Acid Conformation
- Photosynthetic Reaction Center Complex Proteins
- Photosystem II Protein Complex
- RNA Splicing
- RNA, Chloroplast
- RNA, Ribosomal, 23S