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Article

Nuclear genes that promote splicing of group I introns in the chloroplast 23S rRNA and psbA genes in Chlamydomonas reinhardtii.

The Plant journal : for cell and molecular biology - 1 Nov 2002

Li Fei, Holloway Stephen P, Lee Jaesung, Herrin David L

Abstract excerpt

Single nucleotide substitutions were made in the core helices P4, P6, and P7, and in the metal-binding GAAA motif in the J4/5 region of the chloroplast group I rRNA intron of Chlamydomonas reinhardtii, Cr.LSU. In vitro assays showed that these substitutions had surprisingly strong effects on Cr.LSU self-splicing; however, splicing of all but the P6 mutations could be at least partially recovered by increasing the...

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PMID: 12445119
DOI: 10.1046/j.1365-313x.2002.01437.x

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