The Saccharomyces cerevisiae CDC25 gene product binds specifically to catalytically inactive ras proteins in vivo.

doi:10.1128/mcb.12.5.2091-2099.1992

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Article

The Saccharomyces cerevisiae CDC25 gene product binds specifically to catalytically inactive ras proteins in vivo.

Molecular and cellular biology - 1 May 1992

Munder T, Fürst P

Abstract excerpt

Genetic data suggest that the yeast cell cycle control gene CDC25 is an upstream regulator of RAS2. We have been able to show for the first time that the guanine nucleotide exchange proteins Cdc25 and Sdc25 from Saccharomyces cerevisiae bind directly to their targets Ras1 and Ras2 in vivo. Using the characteristics of the yeast Ace1 transcriptional activator to probe for protein-protein interaction, we found that...

Topics

Amino Acid Sequence
Base Sequence
Cell Cycle Proteins
Cloning, Molecular
Escherichia coli
Fungal Proteins
GTP-Binding Proteins
GTPase-Activating Proteins
Genes, Fungal
Genes, Regulator
Genotype

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Molecular Sequence Data

Mutagenesis, Site-Directed

Oligodeoxyribonucleotides

Plasmids

Protein Binding

Recombinant Fusion Proteins

Repressor Proteins

Source

Open in PubMed Open full text

Identifiers

PMID: 1569942
DOI: 10.1128/mcb.12.5.2091-2099.1992
PMCID: PMC364380

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1569942-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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The Saccharomyces cerevisiae CDC25 gene product binds specifically to catalytically inactive ras proteins in vivo.PMID 1569942Read ↗

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