Article
Intergenic suppression in a beta subunit mutant with defective assembly in Escherichia coli F1ATPase. Second-site mutation in the alpha subunit.
FEBS letters - 16 May 1994
Miki J, Tsugumi S, Ikeda H, Kanazawa H
Abstract excerpt
Substitution of Leu-40 by Pro in the beta subunit (beta L40P) of Escherichia coli F1-ATPase caused a decrease in the amount of the alpha and beta subunits on the membranes. A revertant strain, Re50, carrying no suppression mutations in the uncD gene encoding the beta subunit, was isolated from the beta L40P mutant. The uncA gene from this revertant was amplified by PCR, and cloned into an expression plasmid. The...
Topics
- Base Sequence
- Cloning, Molecular
- Escherichia coli
- Macromolecular Substances
- Molecular Sequence Data
- Mutation
- Plasmids
- Polymerase Chain Reaction
- Proton-Translocating ATPases
- Restriction Mapping