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Article

Mutations in alpha-subunit of Escherichia coli F1-ATPase obtained by hydroxylamine-mutagenesis of plasmids carrying the uncA gene.

Archives of biochemistry and biophysics - 1 Mar 1990

Pagan J, Senior A E

Abstract excerpt

In order to generate mutants randomly in the Escherichia coli uncA gene (encoding the alpha-subunit of F1-ATPase), plasmids carrying uncA were treated in vitro with hydroxylamine. Restriction fragments of the mutated uncA gene were then reconstructed into plasmid pDP34, which expresses all of the F1F0 structural genes, and the reconstructed mutant plasmids were expressed in a strain carrying a deletion of...

Topics

Amino Acid Sequence
Cell Membrane
Escherichia coli
Genes, Bacterial
Genotype
Hydroxylamine
Hydroxylamines
Macromolecular Substances
Molecular Sequence Data
Mutation
Plasmids

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Protein Conformation

Proton-Translocating ATPases

Restriction Mapping

Temperature

Source

Identifiers

PMID: 2138002
DOI: 10.1016/0003-9861(90)90580-r

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2138002-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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