Article
Mutations within the uncE gene affecting assembly of the F1F0-ATPase of Escherichia coli.
The Biochemical journal - 15 Jul 1990
Fimmel A L, Karp P E, Norris U
Abstract excerpt
Site-directed mutagenesis has been used to construct two mutations within the uncE gene, which codes for the c-subunit of the F1F0-ATPase, resulting in the substitution of glycine-27 by leucine and of glycine-32 by leucine. Strains carrying each mutation are unable to grow on minimal medium containing succinate as the sole carbon source and possess an uncoupled growth yield. Membranes prepared from strains...
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