Article
Mutations within the uncE gene affecting assembly of the F1F0-ATPase of Escherichia coli.
The Biochemical journal - 15 Jul 1990
Fimmel A L, Karp P E, Norris U
Abstract excerpt
Site-directed mutagenesis has been used to construct two mutations within the uncE gene, which codes for the c-subunit of the F1F0-ATPase, resulting in the substitution of glycine-27 by leucine and of glycine-32 by leucine. Strains carrying each mutation are unable to grow on minimal medium conta...
Topics
- Amino Acid Sequence
- Base Sequence
- Electrophoresis, Gel, Two-Dimensional
- Escherichia coli
- Fluorescence
- Genes, Bacterial
- Glycine
- Leucine
- Molecular Sequence Data
- Mutation
- Plasmids
- Proton-Translocating ATPases
- Quinacrine
- Transformation, Bacterial
