Article
Catalytic site of F1-ATPase of Escherichia coli. Lys-155 and Lys-201 of the beta subunit are located near the gamma-phosphate group of ATP in the presence of Mg2+.
The Journal of biological chemistry - 25 Mar 1991
Ida K, Noumi T, Maeda M, Fukui T, Futai M
Abstract excerpt
The catalytic site of Escherichia coli F1 was probed using a reactive ATP analogue, adenosine triphosphopyridoxal (AP3-PL). For complete loss of enzyme activity, about 1 mol of AP3-PL bound to 1 mol of F1 was estimated to be required in the presence or absence of Mg2+. About 70% of the label was bound to the alpha subunit and the rest to the beta subunit in the absence of Mg2+, and the alpha Lys-201 and beta...
Topics
- Amino Acid Sequence
- Base Sequence
- Catalysis
- Chromatography, High Pressure Liquid
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Lysine
- Magnesium
- Molecular Sequence Data
- Mutation