Article
Cell membrane permeabilization and cellular collapse, followed by loss of dehydrogenase activity: early events in tumour necrosis factor-induced cytotoxicity.
Cytokine - 1 Nov 1993
Grooten J, Goossens V, Vanhaesebroeck B, Fiers W
Abstract excerpt
Early events in the cytotoxic response to tumour necrosis factor (TNF) of the murine fibrosarcoma cell lines L929 and WEHI164cl13 were assessed on a cell by cell basis using the fluorescent exclusion dye propidium iodide (PI) and analysis by flow cytometry. The rationale of this approach is based on the exclusion of PI by cells with intact membranes. PI-positive cells appeared within a few hours of TNF treatment...
Topics
- Animals
- Cell Death
- Cell Membrane Permeability
- Cell Size
- Drug Resistance
- Mice
- Mitochondria
- Phenotype
- Recombinant Proteins
- Succinate Dehydrogenase
- Tumor Cells, Cultured