Article
The mutant DnaAcos protein which overinitiates replication of the Escherichia coli chromosome is inert to negative regulation for initiation.
The Journal of biological chemistry - 2 Sept 1994
Katayama T
Abstract excerpt
Initiation of chromosome replication occurs excessively in the dnaAcos mutant at 30 degrees C. DnaAcos protein was purified from an overproducing strain and found to be as active as wild-type DnaA protein in initial synthesis rates of minichromosome replication in vitro at 30 degrees C. However,...
Topics
- Adenosine Diphosphate
- Adenosine Triphosphate
- Bacterial Proteins
- Chromosomes, Bacterial
- Cold Temperature
- DNA Replication
- DNA, Bacterial
- DNA-Binding Proteins
- Escherichia coli
- Mutation
- Protein Binding