Article
A single amino acid substitution (Glu134-->Ala) in NhaR1 increases the inducibility by Na+ of the product of nhaA, a Na+/H+ antiporter gene in Escherichia coli.
The EMBO journal - 15 Apr 1994
Carmel O, Dover N, Rahav-Manor O, Dibrov P, Kirsch D, Karpel R, Schuldiner S, Padan E
Abstract excerpt
The mutation nhaAup (antup) has now been identified as a Glu134 to Ala substitution in NhaR and designated nhaR1. This was demonstrated by sequence analysis showing that the mutant contains a wild-type nhaA but nhaR1 instead of nhaR and by the finding that nhaR1 cloned in a plasmid confers the NhaAup phenotype. Na+ (107 mM) increases by 5- to 10-fold the level of nhaA transcripts, similar to the effect on the...
Topics
- Amino Acid Sequence
- Bacterial Proteins
- Base Sequence
- DNA-Binding Proteins
- Dose-Response Relationship, Drug
- Enzyme Induction
- Escherichia coli
- Escherichia coli Proteins
- Gene Expression Regulation, Bacterial
- Molecular Sequence Data