A point mutation (G338S) and its suppressor mutations affect both the pH response of the NhaA-Na+/H+ antiporter as well as the growth phenotype of Escherichia coli.

doi:10.1074/jbc.273.41.26470

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Article

A point mutation (G338S) and its suppressor mutations affect both the pH response of the NhaA-Na+/H+ antiporter as well as the growth phenotype of Escherichia coli.

The Journal of biological chemistry - 9 Oct 1998

Rimon A, Gerchman Y, Kariv Z, Padan E

Abstract excerpt

pH controls the activity of the NhaA Na+/H+ antiporter of Escherichia coli. In the present work we show that replacement of glycine 338 of NhaA with serine (G338S) alleviates the pH control of the antiporter. Monitoring Na+-dependent collapse of DeltapH, to assess antiporter activity in isolated...

Topics

Cell Membrane
Escherichia coli
Escherichia coli Proteins
Hydrogen-Ion Concentration
Phenotype
Point Mutation
Sodium-Hydrogen Exchangers
Trypsin

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