Article
MH1, a second-site revertant of an Escherichia coli mutant lacking Na+/H+ antiporters (delta nhaA delta nhaB), regains Na+ resistance and a capacity to excrete Na+ in a delta microH(+)-independent fashion.
The Journal of biological chemistry - 24 Feb 1995
Harel-Bronstein M, Dibrov P, Olami Y, Pinner E, Schuldiner S, Padan E
Abstract excerpt
The Escherichia coli mutant delta nhaA delta nhaB (EP432), which lacks the two specific Na+/H+ antiporter genes, is incapable of efficiently excreting Na+. Accordingly at low K+ (6 mM) medium, its intracellular Na+ concentration is only slightly lower (1.5-2x) than the extracellular concentration (50 mM), explaining the high sensitivity to Na+ (> or = 30 mM) of the mutant. This Na+ sensitivity is shown to be a...
Topics
- Biological Transport
- Escherichia coli
- Genetic Linkage
- Lithium
- Membrane Potentials
- Oxygen
- Phenotype
- Sodium
- Sodium-Hydrogen Exchangers