Cloning and characterization of the choline acetyltransferase structural gene (cha-1) from C. elegans.

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Article

Cloning and characterization of the choline acetyltransferase structural gene (cha-1) from C. elegans.

The Journal of neuroscience : the official journal of the Society for Neuroscience - 1 Apr 1994

Alfonso A, Grundahl K, McManus J R, Rand J B

Abstract excerpt

We have cloned the cha-1 gene from Caenorhabditis elegans using the method of transposon tagging, cha-1 is the structural gene for ChAT, the enzyme that synthesizes ACh. Sequence analysis of cDNAs predicts a protein of 71.5 kDa; comparison of the deduced amino acid sequence with ChAT sequences from other species confirms that cha-1 encodes ChAT. Comparison of cDNA and genomic sequences reveals that transcription...

Topics

Amino Acid Sequence
Animals
Base Sequence
Blotting, Southern
Caenorhabditis elegans
Choline O-Acetyltransferase
Cloning, Molecular
Consensus Sequence
DNA
Drosophila
Exons

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Molecular Sequence Data

Mutagenesis, Insertional

Phenotype

Restriction Mapping

Sequence Homology, Amino Acid

Swine

Transcription, Genetic

Source

Open in PubMed Open full text

Identifiers

PMID: 8158270
PMCID: PMC6577154

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-8158270-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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