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Article

Drosophila choline acetyltransferase temperature-sensitive mutants.

Neurochemical research - 1 Aug 1999

Wang W, Kitamoto T, Salvaterra P M

Abstract excerpt

We used the reverse transcription-polymerase chain reaction (RT-PCR) to amplify choline acetyltransferase (ChAT) mRNA fragments from two temperature-sensitive alleles of Drosophila melanogaster, Cha(ts1) and Cha(ts2). Single base substitutions in the mutants (T1614A in Cha(ts1) and G1596A in Cha(ts2)) would result in amino acid changes for ChAT protein (Met403Lys in Ch(ts1) and Arg397His in Cha(ts2)). These base...

Topics

Alleles
Animals
Base Sequence
Choline O-Acetyltransferase
DNA Primers
Drosophila
Heterozygote
Homozygote
Mutation
RNA, Messenger
Receptors, Cholinergic
Synaptic Transmission

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Temperature

Xenopus

Source

Identifiers

PMID: 10478949
DOI: 10.1023/a:1021021213625

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