Article
Evidence that residues -15 to -46 of the haemolysin secretion signal are involved in early steps in secretion, leading to recognition of the translocator.
Molecular microbiology - 1 Jan 1994
Kenny B, Chervaux C, Holland I B
Abstract excerpt
We previously identified three well-dispersed mutations, E978-K, F989-L and D1009-R within the haemolysin A signal region, located at positions -46, -35 and -15, with respect to the C-terminus, respectively. Each mutation reduces the efficiency of secretion two- to threefold leaving 30-45% of the...
Topics
- Amino Acid Sequence
- Antibodies, Bacterial
- Bacterial Proteins
- Bacterial Toxins
- Blotting, Western
- Carrier Proteins
- Escherichia coli
- Escherichia coli Proteins
- Genes, Bacterial
- Hemolysin Proteins
- Molecular Sequence Data
- Mutation
- Protein Sorting Signals
- Recombinant Fusion Proteins
- Structure-Activity Relationship