Article
Translocation and compartmentalization of Escherichia coli hemolysin (HlyA).
Journal of bacteriology - 1 Jul 1990
Oropeza-Wekerle R L, Speth W, Imhof B, Gentschev I, Goebel W
Abstract excerpt
Hemolysin plasmids were constructed with mutations in hlyB, hlyD, or both transport genes. The localization of hemolysin activity and HlyA protein in these mutants was analyzed by biochemical and immunological methods. It was found that mutants defective in hlyB accumulated internal hemolysin, part of which was associated with the inner membrane and was degraded in the late logarithmic growth phase. In an HlyB+...
Topics
- Enzyme-Linked Immunosorbent Assay
- Escherichia coli
- Freeze Fracturing
- Genes, Bacterial
- Hemolysin Proteins
- Hemolysis
- Immunoblotting
- Microscopy, Electron
- Mutation
- Plasmids