Article
Analysis of the in vivo activation of hemolysin (HlyA) from Escherichia coli.
Journal of bacteriology - 1 Sept 1996
Ludwig A, Garcia F, Bauer S, Jarchau T, Benz R, Hoppe J, Goebel W
Abstract excerpt
Hemolysin (HlyA) from Escherichia coli containing the hlyCABD operon separated from the nonhemolytic pro-HlyA upon two-dimensional (2-D) polyacrylamide gel electrophoresis. The migration distance indicated a net loss of two positive charges in HlyA as a result of the HlyC-mediated activation (modification). HlyA activated in vitro in the presence of [U-14C]palmitoyl-acyl carrier protein comigrated with in...
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