Article
On the rate of proton exchange with solvent of the catalytic histidine in flavocytochrome b2 (yeast L-lactate dehydrogenase).
Protein science : a publication of the Protein Society - 1 Jan 1994
Balme A, Lederer F
Abstract excerpt
The family of FMN-dependent, alpha-hydroxy acid-oxidizing enzymes catalyzes substrate dehydrogenation by a mechanism the first step of which is abstraction of the substrate alpha-proton (so-called carbanion mechanism). For flavocytochrome b2 and lactate oxidase, it was shown that once on the enzy...
Topics
- Crystallization
- Electrochemistry
- Escherichia coli
- Histidine
- Hydrogen Bonding
- Kinetics
- L-Lactate Dehydrogenase
- L-Lactate Dehydrogenase (Cytochrome)
- Molecular Structure
- Mutation
- Protons
- Pyruvates
- Recombinant Proteins
- Saccharomyces cerevisiae
- Solvents