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Article

On the role of Brønsted catalysis in Pseudomonas fluorescens mannitol 2-dehydrogenase.

The Biochemical journal - 1 Oct 2003

Klimacek Mario, Kavanagh Kathryn L, Wilson David K, Nidetzky Bernd

Abstract excerpt

X-ray structure of the Pseudomonas fluorescens mannitol 2-dehydrogenase ternary complex with NAD+ and D-mannitol suggests that Lys-295 provides catalytic base assistance to secondary alcohol group oxidation. We have replaced Lys-295 by site-directed mutagenesis with alanine or methionine and evaluated the catalytic significance of side-chain substitution by kinetic analysis of restoration of activity with...

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Identifiers

PMID: 12826012
DOI: 10.1042/BJ20030733
PMCID: PMC1223657

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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