Article
New strategy for detection of ALDH2 mutant.
Alcohol and alcoholism (Oxford, Oxfordshire). Supplement - 1 Jan 1993
Harada S, Zhang S
Abstract excerpt
Genetic polymorphism of the ALDH2 gene was analysed according to a new technique designated as amplification created restriction site (ACRS). A restriction site for the enzyme (Mbo II) was artificially created in the amplification product using an oligonucleotide primer possessing one mismatched base. Analysis using ACRS technique concerning 41 healthy controls and 63 alcoholics with liver diseases confirmed that...
Topics
- Aldehyde Dehydrogenase
- Alleles
- Base Sequence
- DNA
- DNA Primers
- Humans
- Liver Cirrhosis, Alcoholic
- Molecular Sequence Data
- Oligonucleotides, Antisense
- Point Mutation
- Polymerase Chain Reaction