Article
Disease activity of hepatitis C correlates with single-stage polymerase chain reaction detection of hepatitis C virus.
American journal of clinical pathology - 1 Mar 1994
Finkelstein S D, Sayegh R, Uchman S, Christensen S, Swalsky P A, Bodenheimer H
Abstract excerpt
Hepatitis C virus (HCV) RNA was amplified and detected in the serum of 17 anti-HCV antibody positive patients using a single-stage (30 cycles) polymerase chain reaction (PCR). Specific amplification, targeted to the C-100 protein (anti-HCV) region, was confirmed by direct sequencing of the PCR pr...
Topics
- Adult
- Alanine Transaminase
- Base Sequence
- DNA, Viral
- Female
- Hepacivirus
- Hepatitis C
- Humans
- Male
- Middle Aged
- Molecular Sequence Data
- Mutation
- Polymerase Chain Reaction
- RNA, Viral
- Sequence Analysis, DNA