Article
Disease activity of hepatitis C correlates with single-stage polymerase chain reaction detection of hepatitis C virus.
American journal of clinical pathology - 1 Mar 1994
Finkelstein S D, Sayegh R, Uchman S, Christensen S, Swalsky P A, Bodenheimer H
Abstract excerpt
Hepatitis C virus (HCV) RNA was amplified and detected in the serum of 17 anti-HCV antibody positive patients using a single-stage (30 cycles) polymerase chain reaction (PCR). Specific amplification, targeted to the C-100 protein (anti-HCV) region, was confirmed by direct sequencing of the PCR product. Single-stage PCR detected the virus in 11 patients. Polymerase chain reaction-positive patients demonstrated a...
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