Article
Nucleotide sequence analysis of natural and combinatorial anti-PDC-E2 antibodies in patients with primary biliary cirrhosis. Recapitulating immune selection with molecular biology.
Journal of immunology (Baltimore, Md. : 1950) - 1 Mar 1994
Pascual V, Cha S, Gershwin M E, Capra J D, Leung P S
Abstract excerpt
We have analyzed at the nucleotide level the variable region gene sequences of five human mAbs and five recombinant Fab fragments derived from the mesenteric lymph nodes of patients with primary biliary cirrhosis. Both mAbs and Fabs were monospecific for dihydrolipoamide acetyltransferase, the E2...
Topics
- Amino Acid Sequence
- Antibodies, Monoclonal
- Antibody Diversity
- Autoantibodies
- Autoantigens
- Base Sequence
- DNA Primers
- DNA, Complementary
- Humans
- Immunoglobulin Fab Fragments
- Immunoglobulin Heavy Chains
- Immunoglobulin Variable Region
- Liver Cirrhosis, Biliary
- Molecular Sequence Data