Article
Evidence for distinct locations for metal binding sites in two closely related class I tRNA synthetases.
Journal of biomolecular structure & dynamics - 1 Dec 1993
Schimmel P, Landro J A, Schmidt E
Abstract excerpt
Of the ten class I tRNA synthetases, those for methionine and isoleucine are among the most closely related. In recent work we showed that the 676 amino acid E. coli methionine tRNA synthetase has one zinc bound per polypeptide. Zinc may be replaced by spectroscopically observable cobalt with retention of full activity. Bound zinc has been localized to a cysteine cluster within an insertion into the nucleotide...
Topics
- Amino Acid Sequence
- Binding Sites
- Cobalt
- Enzyme Stability
- Escherichia coli
- Isoleucine-tRNA Ligase
- Methionine-tRNA Ligase
- Molecular Sequence Data
- Mutation
- Oxidation-Reduction
- Protein Structure, Secondary