Article
The invariant arginine in motif 2 of Escherichia coli alanyl-tRNA synthetase is important for catalysis but not for substrate binding.
The Journal of biological chemistry - 22 Apr 1994
Lu Y, Hill K A
Abstract excerpt
Structural motifs 2 and 3, located in the active site of class II aminoacyl-tRNA synthetases, each contain an invariant arginine thought to participate in interactions with ATP. For Escherichia coli alanyl-tRNA synthetase (AlaRS), sequence comparisons indicate that Arg-69 should be aligned with t...
Topics
- Alanine-tRNA Ligase
- Amino Acid Sequence
- Arginine
- Binding Sites
- Blotting, Western
- Catalysis
- Escherichia coli
- Humans
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Phenotype
- Point Mutation
- Protein Structure, Secondary
- Recombinant Proteins
- Saccharomyces cerevisiae
- Sequence Homology, Amino Acid