Article
Complementation of an Arabidopsis thaliana mutant that lacks complex asparagine-linked glycans with the human cDNA encoding N-acetylglucosaminyltransferase I.
Proceedings of the National Academy of Sciences of the United States of America - 1 Mar 1994
Gomez L, Chrispeels M J
Abstract excerpt
N-Acetylglucosaminyltransferase I (EC 2.4.1.101) initiates the conversion of high-mannose asparagine-linked glycans to complex asparagine-linked glycans in plant as well as in animal cells. This Golgi enzyme is missing in the cgl mutant of Arabidopsis thaliana, and the mutant cells are unable to...
Topics
- Arabidopsis
- Asparagine
- Carbohydrate Sequence
- DNA, Complementary
- Genetic Complementation Test
- Humans
- Molecular Sequence Data
- Mutation
- N-Acetylglucosaminyltransferases
- Phenotype
- Plants, Genetically Modified
- Polysaccharides
- Subcellular Fractions
- Substrate Specificity
- Transformation, Genetic