Article
Complementation of the Chlamydomonas reinhardtii arg7-8 (arg2) point mutation by recombination with a truncated nonfunctional ARG7 gene.
Protist - 1 Oct 2007
Mages Wolfgang, Heinrich Oliver, Treuner Gerda, Vlcek Daniel, Daubnerova Ivana, Slaninova Miroslava
Abstract excerpt
Chlamydomonas reinhardtii arg7-8 (arg2) mutant strains carrying a hitherto undescribed mutation in their argininosuccinate lyase gene (ARG7) that leads to arginine auxotrophy have been used together with the corresponding wild-type gene as a very reliable transformation system since 1989. In this study, we finally identify the molecular nature of the arg7-8 mutation as a (6073)G to A transition in exon 9 of ARG7...
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